Wide-range protein photo-crosslinking achieved by a genetically encoded N(ε)-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety.
نویسندگان
چکیده
A derivative of N(ε)-benzyloxycarbonyl-L-lysine with a photo-reactive diazirinyl group, N(ε)-[((4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)oxy)carbonyl]-L-lysine, was site-specifically incorporated into target proteins in mammalian cells. The incorporated photo-crosslinker is able to react not only with residues as distant as about 15 Å but also with those in closer proximity, thus enabling "wide-range" photo-crosslinking of proteins.
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عنوان ژورنال:
- Molecular bioSystems
دوره 8 4 شماره
صفحات -
تاریخ انتشار 2012